I'm relatively new to in-depth NGS experimentation, but am highly experienced in nucleic acid work, focusing mainly on PCR applications (qPCR, RT-qPCR, dPCR, ddPCR, RT-dPCR). I'm currently working on NGS library generation protocols and am getting to grips with the multitude of NGS options from library prep kits and adapters to sequencing platforms and bioinformatics. I look forward to tapping into the wealth of knowledge here and to hopefully gaining enough experience to offer valuable advice myself!
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM