Experimental method for determination of GC content of Genome

[huma Asif]

Dear All,
Can any body help me with any experimental method for determining GC content of a draft and complete bacterial genome beside using bioinformatics tools

Regards

[seb567]

Dear All,
Can any body help me with any experimental method for determining GC content of a draft and complete bacterial genome beside using bioinformatics tools

Regards

I think the obvious experimental method, in my opinion, is to sequence the DNA and use bioinformatics tools.

In EMBOSS, there is a tool called geecee.

http://emboss.sourceforge.net/apps/release/6.3/emboss/apps/geecee.html

I created a Python script called gc-percentage.py

http://pastebin.com/raw.php?i=rSJKq0Pa

just save the content to gc-percentage.py and

./gc-percentage.py genome.fasta > genome.gc