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  • Experimental method for determination of GC content of Genome

    Dear All,
    Can any body help me with any experimental method for determining GC content of a draft and complete bacterial genome beside using bioinformatics tools

    Regards

  • #2
    Originally posted by huma Asif View Post
    Dear All,
    Can any body help me with any experimental method for determining GC content of a draft and complete bacterial genome beside using bioinformatics tools

    Regards
    I think the obvious experimental method, in my opinion, is to sequence the DNA and use bioinformatics tools.

    In EMBOSS, there is a tool called geecee.





    I created a Python script called gc-percentage.py



    just save the content to gc-percentage.py and

    Code:
    ./gc-percentage.py genome.fasta > genome.gc

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