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  • eni86
    Junior Member
    • May 2019
    • 3

    Just to say hello

    Hi
    My name is Jenifer Garcia-Montejo Barneche and I'm a bachellor degree in Biology Sciences.

    Back in 2010 I've studied how to process data from 454, ilumina and ION torrent, and in the biginnings of 2018 did a course called Introduction to Medical Genomics here in Uruguay.

    In the interim I held a teacher position in diferent highschools and now I'm back to finish my Master degree and PhD, so I'm going to be asking questions related to sequencing a lot (I hope)

    So please, wish me luck, and pardon me if I make gammar mistakes

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  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
    07-01-2026, 11:43 AM
  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM

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