*HELP with RNA seq data*
I have a question about Log(2) change 🤓I understand that this some measure of the change in transcript levels when comparing 2 treatments and is a ratio between the 2 treatment groups. What I cannot understand though is how to interpret the numbers. A log(2) fold change of more than 1 means enrichment (I think) and a negative log(2) fold enrichment is lower transcript levels (I think). Since log(2) fold change is a ratio, what does a log(2) change that is a positive value but less than 1 (+0 to 0.99) mean?
In an attempt to clarify my question: I have a table of log(2) fold changes that I am trying to interpret. I think a log(2) fold change of 2.2 = enrichment; a log(2) fold change of -0.33 is lower transcript levels (is this a correct interpretation?). What is a log(2) fold change of 0.67?
Any help is much appreciated
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The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...07-08-2024, 03:19 PM