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  • Sequencing PCR products

    Hello to all,

    I am trying to find information about sequencing pcr products that I made from my RNA transcript. I am wondering if I could use it directly for sequencing using Illumina or other Next-gen sequencing.
    My transcript size is around 100-200.
    Also, if anyone could tell me about the pricing for sequencing using Illumina ( estimates).

    Thank you in advance.

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  • seqadmin
    Improved Targeted Sequencing: A Comprehensive Guide to Amplicon Sequencing
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    Amplicon sequencing is a targeted approach that allows researchers to investigate specific regions of the genome. This technique is routinely used in applications such as variant identification, clinical research, and infectious disease surveillance. The amplicon sequencing process begins by designing primers that flank the regions of interest. The DNA sequences are then amplified through PCR (typically multiplex PCR) to produce amplicons complementary to the targets. RNA targets...
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  • seqadmin
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    Targeted sequencing is an effective way to sequence and analyze specific genomic regions of interest. This method enables researchers to focus their efforts on their desired targets, as opposed to other methods like whole genome sequencing that involve the sequencing of total DNA. Utilizing targeted sequencing is an attractive option for many researchers because it is often faster, more cost-effective, and only generates applicable data. While there are many approaches...
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