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Characterization and improvement of RNA-Seq precision in quantitative transcript expression profiling.
Bioinformatics. 2011 Jul 1;27(13):i383-i391
Authors: Labaj PP, Leparc GG, Linggi BE, Markillie LM, Wiley HS, Kreil DP
Measurement precision determines the power of any analysis to reliably identify significant signals, such as in screens for differential expression, independent of whether the experimental design incorporates replicates or not. With the compilation of large-scale RNA-Seq datasets with technical replicate samples, however, we can now, for the first time, perform a systematic analysis of the precision of expression level estimates from massively parallel sequencing technology. This then allows considerations for its improvement by computational or experimental means.
PMID: 21685096 [PubMed - in process]
More...
Characterization and improvement of RNA-Seq precision in quantitative transcript expression profiling.
Bioinformatics. 2011 Jul 1;27(13):i383-i391
Authors: Labaj PP, Leparc GG, Linggi BE, Markillie LM, Wiley HS, Kreil DP
Measurement precision determines the power of any analysis to reliably identify significant signals, such as in screens for differential expression, independent of whether the experimental design incorporates replicates or not. With the compilation of large-scale RNA-Seq datasets with technical replicate samples, however, we can now, for the first time, perform a systematic analysis of the precision of expression level estimates from massively parallel sequencing technology. This then allows considerations for its improvement by computational or experimental means.
PMID: 21685096 [PubMed - in process]
More...
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