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Sample preparation for small RNA massive parallel sequencing.
Methods Mol Biol. 2012;786:167-78
Authors: Gommans WM, Berezikov E
Abstract
High-throughput sequencing has allowed for a comprehensive small RNA (sRNA) expression analysis of numerous tissues in a diverse set of organisms. The computational analysis of the millions of generated sequencing reads has led to the discovery of novel miRNAs and other sRNA species, and resulted in a better understanding of the roles these sRNAs play in development and disease. This chapter describes the generation of sRNA deep-sequencing libraries for the Illumina massively parallel sequencing platform by using a cloning method that anneals specific RNA sequences to the 5'- and 3'-ends of the sRNA molecules.
PMID: 21938626 [PubMed - in process]
More...
Sample preparation for small RNA massive parallel sequencing.
Methods Mol Biol. 2012;786:167-78
Authors: Gommans WM, Berezikov E
Abstract
High-throughput sequencing has allowed for a comprehensive small RNA (sRNA) expression analysis of numerous tissues in a diverse set of organisms. The computational analysis of the millions of generated sequencing reads has led to the discovery of novel miRNAs and other sRNA species, and resulted in a better understanding of the roles these sRNAs play in development and disease. This chapter describes the generation of sRNA deep-sequencing libraries for the Illumina massively parallel sequencing platform by using a cloning method that anneals specific RNA sequences to the 5'- and 3'-ends of the sRNA molecules.
PMID: 21938626 [PubMed - in process]
More...