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Strategy for modular tagged high-throughput amplicon sequencing.
Appl Environ Microbiol. 2011 Sep;77(17):6310-2
Authors: de Cárcer DA, Denman SE, McSweeney C, Morrison M
Abstract
The use and validation of a strategy that allows a universal set of bar-coded sequencing primers to be appended to an amplified PCR product is described. The strategy allows a modular approach, in that the same bar code can be used with two or more target-specific primer sets, even simultaneously.
PMID: 21764953 [PubMed - in process]
More...
Strategy for modular tagged high-throughput amplicon sequencing.
Appl Environ Microbiol. 2011 Sep;77(17):6310-2
Authors: de Cárcer DA, Denman SE, McSweeney C, Morrison M
Abstract
The use and validation of a strategy that allows a universal set of bar-coded sequencing primers to be appended to an amplified PCR product is described. The strategy allows a modular approach, in that the same bar code can be used with two or more target-specific primer sets, even simultaneously.
PMID: 21764953 [PubMed - in process]
More...