N Engl J Med. 2009 Jun 25;360(26):2719-29. Epub 2009 Jun 10.
Mutation of FOXL2 in granulosa-cell tumors of the ovary.
It just published online by vancouver group. They found the novel gene FOXL2 mutation in granulosa tumor with Pair end sequencing.
However, I have question about the Table 1.
(1) Why some library the mapped reads are more than the total reads?
(2) Why the ratio of sequence reads of intronic and intergenic are so high in transcriptome study(intronic 14~39%, intergenic 10~17%)?
Mutation of FOXL2 in granulosa-cell tumors of the ovary.
It just published online by vancouver group. They found the novel gene FOXL2 mutation in granulosa tumor with Pair end sequencing.
However, I have question about the Table 1.
(1) Why some library the mapped reads are more than the total reads?
(2) Why the ratio of sequence reads of intronic and intergenic are so high in transcriptome study(intronic 14~39%, intergenic 10~17%)?