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A transposable approach to RNA-seq from total RNA.
Nat Methods. 2012 May 30;9(6)
Authors: Kuersten S
Abstract
The rapid progress of RNA-seq technology requires the development of simple, versatile tools that competently reflect RNA levels in the cell. We have developed the TotalScript™ RNA-Seq Kit, a new, transposome-based directional library prep method suitable for a variety of RNA sample types and a range of input. TotalScript libraries exhibit high complexity, ~97-99% directionality and low rRNA contamination. The method is scalable and robust, allowing input amounts of ~100 pg of poly(A)(+) RNA or ~5 ng of total RNA.
PMID: 22874996 [PubMed - in process]
More...
A transposable approach to RNA-seq from total RNA.
Nat Methods. 2012 May 30;9(6)
Authors: Kuersten S
Abstract
The rapid progress of RNA-seq technology requires the development of simple, versatile tools that competently reflect RNA levels in the cell. We have developed the TotalScript™ RNA-Seq Kit, a new, transposome-based directional library prep method suitable for a variety of RNA sample types and a range of input. TotalScript libraries exhibit high complexity, ~97-99% directionality and low rRNA contamination. The method is scalable and robust, allowing input amounts of ~100 pg of poly(A)(+) RNA or ~5 ng of total RNA.
PMID: 22874996 [PubMed - in process]
More...