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Transcriptomes of the desiccation tolerant resurrection plant Craterostigma plantagineum.
Plant J. 2010 Apr 29;
Authors: Rodriguez MC, Edsgärd D, Sarfraz HS, Alquezar D, Rasmussen M, Gilbert T, Nielsen BH, Bartels D, Mundy J
ABSTRACT Studies of the resurrection plant Craterostigma plantagineum have revealed some of the mechanisms which these desiccation tolerant plants use to survive environments with extreme dehydration and restricted seasonal water. Most resurrection plants are polyploid with large genomes, which has hindered efforts to obtain whole genome sequences and to perform mutational analysis. However, the application of deep sequencing technologies to transcriptomics now permits large scale analyses of gene expression patterns despite the lack of a reference genome. Here we use pyrosequencing to characterize the transcriptomes of C. plantagineum leaves at four stages of dehydration and rehydration. This reveals that genes involved in several pathways, such as those required for vitamin K and thiamin biosynthesis, are tightly regulated at the level of gene expression. Our analysis also provides a comprehensive picture of the array of cellular responses controlled by gene expression that allow resurrection plants to survive desiccation.
PMID: 20444235 [PubMed - as supplied by publisher]
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Transcriptomes of the desiccation tolerant resurrection plant Craterostigma plantagineum.
Plant J. 2010 Apr 29;
Authors: Rodriguez MC, Edsgärd D, Sarfraz HS, Alquezar D, Rasmussen M, Gilbert T, Nielsen BH, Bartels D, Mundy J
ABSTRACT Studies of the resurrection plant Craterostigma plantagineum have revealed some of the mechanisms which these desiccation tolerant plants use to survive environments with extreme dehydration and restricted seasonal water. Most resurrection plants are polyploid with large genomes, which has hindered efforts to obtain whole genome sequences and to perform mutational analysis. However, the application of deep sequencing technologies to transcriptomics now permits large scale analyses of gene expression patterns despite the lack of a reference genome. Here we use pyrosequencing to characterize the transcriptomes of C. plantagineum leaves at four stages of dehydration and rehydration. This reveals that genes involved in several pathways, such as those required for vitamin K and thiamin biosynthesis, are tightly regulated at the level of gene expression. Our analysis also provides a comprehensive picture of the array of cellular responses controlled by gene expression that allow resurrection plants to survive desiccation.
PMID: 20444235 [PubMed - as supplied by publisher]
More...