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Related Articles Methylation-sensitive single-molecule analysis of chromatin structure.
Curr Protoc Mol Biol. 2010 Jan;Chapter 21:Unit 21.17.1-16
Authors: Miranda TB, Kelly TK, Bouazoune K, Jones PA
Methylation-sensitive single-molecule analysis of chromatin structure is a high-resolution method for studying nucleosome positioning. As described in this unit, this method allows for the analysis of the chromatin structure of unmethylated CpG islands or in vitro-remodeled nucleosomes by treatment with the CpG-specific DNA methyltransferase SssI (M.SssI), followed by bisulfite sequencing of individual progeny DNA molecules. Unlike nuclease-based approaches, this method allows each molecule to be viewed as an individual entity instead of an average population.
PMID: 20069538 [PubMed - indexed for MEDLINE]
More...
Related Articles Methylation-sensitive single-molecule analysis of chromatin structure.Curr Protoc Mol Biol. 2010 Jan;Chapter 21:Unit 21.17.1-16
Authors: Miranda TB, Kelly TK, Bouazoune K, Jones PA
Methylation-sensitive single-molecule analysis of chromatin structure is a high-resolution method for studying nucleosome positioning. As described in this unit, this method allows for the analysis of the chromatin structure of unmethylated CpG islands or in vitro-remodeled nucleosomes by treatment with the CpG-specific DNA methyltransferase SssI (M.SssI), followed by bisulfite sequencing of individual progeny DNA molecules. Unlike nuclease-based approaches, this method allows each molecule to be viewed as an individual entity instead of an average population.
PMID: 20069538 [PubMed - indexed for MEDLINE]
More...