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Structural shifts of gut microbiota as surrogate endpoints for monitoring host health changes induced by carcinogen exposure.
FEMS Microbiol Ecol. 2010 Jul 7;
Authors: Wei H, Dong L, Wang T, Zhang M, Hua W, Zhang C, Pang X, Chen M, Su M, Qiu Y, Zhou M, Yang S, Chen Z, Rantalainen M, Nicholson JK, Jia W, Wu D, Zhao L
Abstract This study monitored structural shifts of gut microbiota of rats developing precancerous mucosal lesions induced by carcinogen 1,2-dimethyl hydrazine (DMH) treatment using PCR-denaturing gradient gel electrophoresis (DGGE) and 454 pyrosequencing on the 16S rRNA gene V3 region. Partial least square discriminant analysis of DGGE fingerprints showed that the gut microbiota structure of treated animals was similar to that of the controls 1 and 3 weeks after DMH treatments, but significantly different 7 weeks after DMH treatments, when a large number of aberrant crypt foci (ACF) developed in their colons. Martens' uncertainty test, followed by anova test (P
Structural shifts of gut microbiota as surrogate endpoints for monitoring host health changes induced by carcinogen exposure.
FEMS Microbiol Ecol. 2010 Jul 7;
Authors: Wei H, Dong L, Wang T, Zhang M, Hua W, Zhang C, Pang X, Chen M, Su M, Qiu Y, Zhou M, Yang S, Chen Z, Rantalainen M, Nicholson JK, Jia W, Wu D, Zhao L
Abstract This study monitored structural shifts of gut microbiota of rats developing precancerous mucosal lesions induced by carcinogen 1,2-dimethyl hydrazine (DMH) treatment using PCR-denaturing gradient gel electrophoresis (DGGE) and 454 pyrosequencing on the 16S rRNA gene V3 region. Partial least square discriminant analysis of DGGE fingerprints showed that the gut microbiota structure of treated animals was similar to that of the controls 1 and 3 weeks after DMH treatments, but significantly different 7 weeks after DMH treatments, when a large number of aberrant crypt foci (ACF) developed in their colons. Martens' uncertainty test, followed by anova test (P