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Related Articles Function annotation of rice transcriptome at single nucleotide resolution by RNA-seq.
Genome Res. 2010 Jul 13;
Authors: Lu T, Lu G, Fan D, Zhu C, Li W, Zhao Q, Feng Q, Zhao Y, Guo Y, Li W, Huang X, Han B
The functional complexity of the rice transcriptome is not yet fully elucidated despite many studies having reported the use of DNA microarrays. Next-generation DNA sequencing technologies provide a powerful approach for mapping and quantifying the transcriptome, termed RNA sequencing (RNA-seq). In this study, we applied RNA-seq to globally sample transcripts of the cultivated rice Oryza sativa indica and japonica subspecies for resolving the whole-genome transcription profiles. We identified 15,708 novel transcriptional active regions (nTARs), of which 51.7% have no homolog to public protein data and more than 63% are putative single exon transcripts, which are highly different from protein-coding genes (
Related Articles Function annotation of rice transcriptome at single nucleotide resolution by RNA-seq.
Genome Res. 2010 Jul 13;
Authors: Lu T, Lu G, Fan D, Zhu C, Li W, Zhao Q, Feng Q, Zhao Y, Guo Y, Li W, Huang X, Han B
The functional complexity of the rice transcriptome is not yet fully elucidated despite many studies having reported the use of DNA microarrays. Next-generation DNA sequencing technologies provide a powerful approach for mapping and quantifying the transcriptome, termed RNA sequencing (RNA-seq). In this study, we applied RNA-seq to globally sample transcripts of the cultivated rice Oryza sativa indica and japonica subspecies for resolving the whole-genome transcription profiles. We identified 15,708 novel transcriptional active regions (nTARs), of which 51.7% have no homolog to public protein data and more than 63% are putative single exon transcripts, which are highly different from protein-coding genes (