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Retrospective application of transposon-directed insertion-site sequencing to a library of signature-tagged mini-Tn5Km2 mutants of Escherichia coli O157:H7 screened in cattle.
J Bacteriol. 2011 Jan 28;
Authors: Eckert S, Dziva F, Chaudhuri RR, Langridge GC, Turner DJ, Pickard DJ, Maskell DJ, Thomson NR, Stevens MP
Massively-parallel sequencing of transposon-flanking regions assigned the genotype and fitness score of 91% of Escherichia coli O157:H7 mutants previously screened in cattle by signature-tagged mutagenesis (STM). The method obviates the limitations of STM and markedly extended the functional annotation of the prototype E. coli O157:H7 genome without further animal use.
PMID: 21278291 [PubMed - as supplied by publisher]
More...
Retrospective application of transposon-directed insertion-site sequencing to a library of signature-tagged mini-Tn5Km2 mutants of Escherichia coli O157:H7 screened in cattle.
J Bacteriol. 2011 Jan 28;
Authors: Eckert S, Dziva F, Chaudhuri RR, Langridge GC, Turner DJ, Pickard DJ, Maskell DJ, Thomson NR, Stevens MP
Massively-parallel sequencing of transposon-flanking regions assigned the genotype and fitness score of 91% of Escherichia coli O157:H7 mutants previously screened in cattle by signature-tagged mutagenesis (STM). The method obviates the limitations of STM and markedly extended the functional annotation of the prototype E. coli O157:H7 genome without further animal use.
PMID: 21278291 [PubMed - as supplied by publisher]
More...