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  • KAPA qPCR kit for XL+ libraries?

    Are you successfully quantifying your XL+ libraries with this kit? Have you modified (extended) cycling times?

    I have tried with extended denaturation and extension times and dind´t get very good results...

    Any help?

  • #2
    Hi there,

    Yes I do this. I use a 2 minute extension time and it seems pretty consistent. Good luck!

    Comment


    • #3
      Hi RCJK.
      I tried with 30secs denaturation time and 2 min annealing/extension time and it worked just fine. Thank you!

      Comment

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