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  • razibus
    Member
    • May 2011
    • 25
    #1

    heterogeneity of results

    We are working on sequencing human exome using agilent for enrichment and GAIIx for sequencing.

    We got a high heterogeneity of read profiles from a DNA sample to an other.

    For example : for a given mutation, we got for 1 DNA sample a deph of 50X, for an other it will be 2X and so on.

    Is it a problem due to Agilent or Illumina?

    Did anybody read a paper about this issue?
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