Have you ever read such report? seems I found some sequence error hotspots.
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Error "hotspots" exist for every technology I've looked at.
E.g.:- older Sanger ABIs (370 and 373) had the "AG-problem".
- 454 has the homopolymer problem. To be honest, Sanger sequencing and Solexa also have it ... but much less pronounced.
- for the Solexa projects I've analysed, I've seen strong bias after GGC.G, see also http://seqanswers.com/forums/showthread.php?t=1353
SOLiD I have no idea, I never analysed that data.
Regards,
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Hey Bach, Thanks for your response, we plan to go deep into the sequence error, hope can get more general ideas.
now we get too much AC misidentification, and seems they cluster into several positions.Last edited by mingkunli; 03-19-2009, 07:49 AM.Comment
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I guess that is due to A and C intensities being so similar.. and phasing makes it worse with increasing cycle-number--
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The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.
This convergence of genetics, immunology, and computation...09-01-2026, 05:41 AM
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