In our last couple of runs on our HiScanSQ (which uses HiSeq chemistry/flowcells) we get a cluster density picture that looks like:
(I masked out 3 of the lanes because they had high primer-dimer contamination that threw off the cluster calling.)
Do any of you typically see this much cluster density variation from top to bottom of a lane (around 2x for this run.) More importantly, does anyone know how to keep the gradient to a minimum?
--
Phillip
(I masked out 3 of the lanes because they had high primer-dimer contamination that threw off the cluster calling.)
Do any of you typically see this much cluster density variation from top to bottom of a lane (around 2x for this run.) More importantly, does anyone know how to keep the gradient to a minimum?
--
Phillip
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