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  • papori
    Senior Member
    • Dec 2010
    • 181

    #1

    segmentation fault in BWA sampe

    Hi all,
    i am using bwa-0.5.9 for illumina output.

    i have reads from 2 samples of same organism.
    So i have 2 databases.

    when i run BWA on the first, everything went well. the SAM file is 3.3GB.

    On the second, i got segmentation fault in the last part, when converting to *.sam.
    it succeed to convert 2.2GB , and then seg` fault.

    My steps are:
    bwa index -a is database.fasta

    bwa aln database.fasta read_1.fastq > database_aln_sa_1.sai
    bwa aln database.fasta read_2.fastq > database_aln_sa_2.sai

    bwa sampe database.fasta database_aln_sa_1.sai database_aln_sa_2.sai read_1.fastq read_2.fastq > database_aln.sam

    When i run bwa with samse on each end separately , i got 2 sam file , each in size 1.6GB , as expected.


    My questions are:
    How can i solve the Segmentation fault?
    Or, Can i merge the 2 sam files that have been resulted separately to one sam?without loosing any data?

    Thanks in advance..

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