I have 50 bp Illumina RNA-seq reads that display a strange behaviour.
Per base sequence quality has not the usual trend for Illumina data.
The base with the highest is the number 14, and at the 5' end, quality raises in a step way.
Has anyone seen this behaviour?
Per base sequence quality has not the usual trend for Illumina data.
The base with the highest is the number 14, and at the 5' end, quality raises in a step way.
Has anyone seen this behaviour?
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