Originally posted by Isequencestuff
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As far as some of your final libraries having much higher concentrations than you expected... (Like way above 10nM.) Illumina kits are designed to overkill. They have to be so that samples with lower quality or lower quantity will still produce something you can use. Typically we will take a look at the preamp libraries on the bioanalyzer and usually end up doing only 4 cycles instead of 10.
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Phillip
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