Hello ,
I have to prepare a ChIP Seq library for the first time of my life and I would like to know which kind of control should I do to valid my library ? And the Illumina's protocol recommends to do a QPCR to quantify the sample's DNA, but if I need do sequence my sample, it's because I don't know it, so how can I do a QPCR ... ? I don't understand this step
So, Is there someone who can help me and give me some answers please ??
Sorry for my bad english
frigo
I have to prepare a ChIP Seq library for the first time of my life and I would like to know which kind of control should I do to valid my library ? And the Illumina's protocol recommends to do a QPCR to quantify the sample's DNA, but if I need do sequence my sample, it's because I don't know it, so how can I do a QPCR ... ? I don't understand this step
So, Is there someone who can help me and give me some answers please ??
Sorry for my bad english
frigo
Comment