Thanks guys,
This is the first time for us to convert .bcl to fastq, not too much experience, but soon will improve computing ability; just a little updating what we have and what I am doing, maybe have more good ideas about this process.
We have suffered a lot from installing and running CASAVA before did it; we installed a Linux CentOS using Parallels in a MAC pro machine. The MAC pro is pretty powerful: 2X2.93 GHz 6-Core Intel Xeon, 24GB 1333MHz DDR3, and 5TB drive; however, the virtue machine only allows us use 8 cores and 8 GB memory. However, we have data from 209 cycles of 8 lane from HiSeq machine, and the computer has run like 40 hours, we don't want to stop it and hope it can finish the work next Monday. Of course, after this time, we would like to do the following things to improve computing abilities, however, please have a look and give some advice to feasibilities:
1) wipe off MAC OS and install CentOS--- big concerns here, anybody have been successful in this? can share some experience?
2) if purchase a stronger server, any suggestions?
3) to improve the speed of I/O, connecting hard drives with fiber channel?
thanks for any suggestions and comments!
This is the first time for us to convert .bcl to fastq, not too much experience, but soon will improve computing ability; just a little updating what we have and what I am doing, maybe have more good ideas about this process.
We have suffered a lot from installing and running CASAVA before did it; we installed a Linux CentOS using Parallels in a MAC pro machine. The MAC pro is pretty powerful: 2X2.93 GHz 6-Core Intel Xeon, 24GB 1333MHz DDR3, and 5TB drive; however, the virtue machine only allows us use 8 cores and 8 GB memory. However, we have data from 209 cycles of 8 lane from HiSeq machine, and the computer has run like 40 hours, we don't want to stop it and hope it can finish the work next Monday. Of course, after this time, we would like to do the following things to improve computing abilities, however, please have a look and give some advice to feasibilities:
1) wipe off MAC OS and install CentOS--- big concerns here, anybody have been successful in this? can share some experience?
2) if purchase a stronger server, any suggestions?
3) to improve the speed of I/O, connecting hard drives with fiber channel?
thanks for any suggestions and comments!
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