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Hi,
we are planning our new sequencing experiment and are arguing about single end reads against paired end reads.
If we sequence 100bp PE reads instead of 100bp SE reads it would cost ~70% more. So our/my main question is:
Do you thing it's worth it to sequence PE reads, ie. is the gain in information worth the extra money?
As we have limited human resources, I think analyzing the data will last at least a year. Do you think by then it is cheaper to sequence it with PE reads again as the cost are going down, ie. could we get it done for the same prize = the 70% we are would now pay extra?
Any suggestions would be highly appreciated!!
All the best!
we are planning our new sequencing experiment and are arguing about single end reads against paired end reads.
If we sequence 100bp PE reads instead of 100bp SE reads it would cost ~70% more. So our/my main question is:
Do you thing it's worth it to sequence PE reads, ie. is the gain in information worth the extra money?
As we have limited human resources, I think analyzing the data will last at least a year. Do you think by then it is cheaper to sequence it with PE reads again as the cost are going down, ie. could we get it done for the same prize = the 70% we are would now pay extra?
Any suggestions would be highly appreciated!!
All the best!
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