Can I use same adapters for PE and single read?

[ein_io]

Greetings everyone,

I am a novice and my question would probably seem trivial to most of you

I haven't decided yet whether I need single read or paired-end read sequencing. I would like to start from a single read (cheaper ).

Therefore I wonder whether I can use adapters for Paired End reads for making a library that would be sequenced by single read protocol?

thanks

ein_io

[ETHANol]

Yes use Illumina's TruSeq adapters. You can get the sequences from Illumina tech support.

Or as a beginner you are probably better off buying a kit. The price per library for most kits isn't more then buying the reagents separately. But it depends on your application. Without more specifics there isn't more to be said.

[ein_io]

Thanks for your answer, ETHANol

I used not TruSeq but PE-adapters for my design
Paired End DNA oligonucleotide sequences
PE Adapters
5' P-GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
5' ACACTCTTTCCCTACACGACGCTCTTCCGATCT
PE PCR Primer 1.0
5' AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
PE PCR Primer 2.0
5' CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT
PE Read 1 Sequencing Primer
5' ACACTCTTTCCCTACACGACGCTCTTCCGATCT
PE Read 2 Sequencing Primer
5' CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT

I plan to request reads from ACACTCTTTCCCTACACGACGCTCTTCCGATCT
would that be OK?

[ETHANol]

Yes you can use them for single read sequencing.

The single read sequencing primer is the same as the paired end read 1 sequencing primer.

[ein_io]

Big Thanks, Ethanol!!!