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  • #16
    Originally posted by GenoMax View Post
    The option "--control-lane=X" is to be used with bustard after the run completes. This does require that the lane "X" be marked as "control" during the run (which you have done).
    IMHO this is not necessary; Lane X should look good, that's more important :-) We do this from time to time with chipseq data.

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    • #17
      Originally posted by victorsor View Post
      Probably i do not explained well. A control lane is already defined in the current run.
      If there was a control lane defined then there should be no need to go messing about with matrix or phasing settings (assuming the control lane looked OK).

      If you've still got no clusters then the only remaining remedy would be to run the bclToFastq with the --no-eamss option to see if the qualities temporarily dip and then recover.

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      • #18
        Thank you all. We will try to do your suggestions when run finish. I hope that not all data go to waste...

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