No, because due to the use of an empirical-Bayes estimation method for dispersion estimation, you do not consume DoFs as usual.
Otherwise, it would be completely impossible to perform comparisons between groups with only two to four samples per group. For details, see Gordon Smyth's original 2004 paper on limma, which introduced this whole idea of understanding empirical-Bayes shrinkage estimation as a way of "saving degrees of freedom" by sharing information across genes.
Otherwise, it would be completely impossible to perform comparisons between groups with only two to four samples per group. For details, see Gordon Smyth's original 2004 paper on limma, which introduced this whole idea of understanding empirical-Bayes shrinkage estimation as a way of "saving degrees of freedom" by sharing information across genes.
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