Hello,
This is the first time I am posting here. In our lab, we mostly cluster with a library concentration of 10pM. However, we have a few libraries that we tried to cluster at 10pM but they showed so high cluster densities in the first base reports that we had to abort the run. The concentrations were all fine 'cause we re-ran the libraries on Bioanalyzer to make sure we had the right quantification.
My question is that the concentration of a library on which to cluster it depends on the kind of library as well, what could be specific of a library that decides this concentration. Or in other words, if I have two libraries, and I sequence both at 10pM so that one gives an optimal cluster density while the other is just too high, then what could be difference between the types/make of these two libraries?
Thanks!
This is the first time I am posting here. In our lab, we mostly cluster with a library concentration of 10pM. However, we have a few libraries that we tried to cluster at 10pM but they showed so high cluster densities in the first base reports that we had to abort the run. The concentrations were all fine 'cause we re-ran the libraries on Bioanalyzer to make sure we had the right quantification.
My question is that the concentration of a library on which to cluster it depends on the kind of library as well, what could be specific of a library that decides this concentration. Or in other words, if I have two libraries, and I sequence both at 10pM so that one gives an optimal cluster density while the other is just too high, then what could be difference between the types/make of these two libraries?
Thanks!
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