Hi, there.
We've have experienced puzzling troubles when we tried to analyze a multiplexed sequencing on Illumina HiSeq platform. Something is wrong with our output results, considering that, we had 7 multiplex libraries together and when we tried Cassava demultiplexing we found "contamination" of results.
I mean that reads expected to belong, i.e., to library #1 are spread everywhere, with nonsense relationships.
It's like a big mosaic of untidy correspondences. We tried to change demultiplexing parameters, without any improvement.
What do you think? Where is the key? In bioinformatics, or worse, it is possible that some biological contamination of reagents occurred? That index-seq primers were accidentally mixed?
It seems far option, but if not what else?
Please, help...
We've have experienced puzzling troubles when we tried to analyze a multiplexed sequencing on Illumina HiSeq platform. Something is wrong with our output results, considering that, we had 7 multiplex libraries together and when we tried Cassava demultiplexing we found "contamination" of results.
I mean that reads expected to belong, i.e., to library #1 are spread everywhere, with nonsense relationships.
It's like a big mosaic of untidy correspondences. We tried to change demultiplexing parameters, without any improvement.
What do you think? Where is the key? In bioinformatics, or worse, it is possible that some biological contamination of reagents occurred? That index-seq primers were accidentally mixed?
It seems far option, but if not what else?
Please, help...
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