Hello
I am sequencing a custom made library meant to serve for tag counting.
We configured the machine for pretty long reads (151).
We observe a large number of duplications happening WITHIN individual reads. (e.g. ATCGATGTGATCGT-tagcctagcga-tagcctagcga-tagcctagcga-ACGATGTCGATCGTAG)
We suspect that these duplications happen during the cluster building in a flow cell
I was wondering whether anyone has observed it before and if there are any known ways to fix it
Thanks for your help,
I am sequencing a custom made library meant to serve for tag counting.
We configured the machine for pretty long reads (151).
We observe a large number of duplications happening WITHIN individual reads. (e.g. ATCGATGTGATCGT-tagcctagcga-tagcctagcga-tagcctagcga-ACGATGTCGATCGTAG)
We suspect that these duplications happen during the cluster building in a flow cell
I was wondering whether anyone has observed it before and if there are any known ways to fix it
Thanks for your help,