In the sticky thread "Illumina/Solexa Genome Analyzer Primer/Adapter Sequences", the 3rd reply of horigen tried to make people understand the sequence of adapter better, however, it misleaded people quite much. Actually, I spent almost 6 month to figure out it was totally wrong and find out the truth.
The Oligos provided by Illumina are just the right sequences, you need not order any more complemental oligo as horigen indicated.
The following figure is a schematic I made for preparing single-end sequence sample.
Wish not so many people spent the same time as me on the same mistake.
I also suggest delete the 3rd reply to prevent further misleading.
Kai
The Oligos provided by Illumina are just the right sequences, you need not order any more complemental oligo as horigen indicated.
The following figure is a schematic I made for preparing single-end sequence sample.
Wish not so many people spent the same time as me on the same mistake.
I also suggest delete the 3rd reply to prevent further misleading.
Kai
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