Does anyone have experience combining data taken from runs processed using different versions of CASAVA?
We're looking at a large sequencing project where the version was changed from 1.5 to 1.8 during the course of the experiment. Some of the samples had multiple lanes run with each of the versions. Likewise, some matched samples were run before and after the switch.
We'd like to be able to combine BAM files in the former case, and operate on the matched sets in the latter, but am concerned that the differences in processing software will introduce bias into the results.
Any ideas would be welcome.
Thanks,
Matt
We're looking at a large sequencing project where the version was changed from 1.5 to 1.8 during the course of the experiment. Some of the samples had multiple lanes run with each of the versions. Likewise, some matched samples were run before and after the switch.
We'd like to be able to combine BAM files in the former case, and operate on the matched sets in the latter, but am concerned that the differences in processing software will introduce bias into the results.
Any ideas would be welcome.
Thanks,
Matt
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