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Illumina Cancer Panel problems
We are getting either no or low yield after the PCR when running this assay. One thing we have noticed is that during the long annealing of the initial primers, there is substantial evaporation even using the recommended plates and seals. We switched to strip tubes which fixed this issue, however we continue to get very low yields whether on our samples or the positive controls. Has anyone else had issues like this with either the cancer panel or the custom amplicon panel? We have checked with Illumina technical service but have yet to figure out what is going on. Input would be appreciated.
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...Today, 07:48 AM -
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM