Hello
We would like to use the MiSeq to sequence 8 amplicons in a few thousand samples. I realise that this is a low number of amplicons and this might cause us problems, we have been advised to use 3 primers sets for each amplicons to try to increase the diversity or to spike with 20% PhiX. Illumina doesn't support this sort study as such but has suggested their TruSeq HT kit. I was just wondering if anyone had any suggestions for alternatives? And if anyone could advise on doing this in a homebrew fashion we would be very grateful!
Thanks!
We would like to use the MiSeq to sequence 8 amplicons in a few thousand samples. I realise that this is a low number of amplicons and this might cause us problems, we have been advised to use 3 primers sets for each amplicons to try to increase the diversity or to spike with 20% PhiX. Illumina doesn't support this sort study as such but has suggested their TruSeq HT kit. I was just wondering if anyone had any suggestions for alternatives? And if anyone could advise on doing this in a homebrew fashion we would be very grateful!
Thanks!
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