Hi,
I need to sequence ~50 short amplicon (100-150bp each) from 4 samples. I want to bar-code each sample so I could sequence all of them in one lane. I have a thought about simplified way of bar coding and not sure whether it would work. Could I add the 6 base barcode at the 5' of my forward primers? In that case, I would be able to know which sample the sequence is from just by looking at the reading from the first 6 bases? Does anyone know why illumina design the third run of sequencing just to get the sequence of the barcodes? Thanks.
Jim
I need to sequence ~50 short amplicon (100-150bp each) from 4 samples. I want to bar-code each sample so I could sequence all of them in one lane. I have a thought about simplified way of bar coding and not sure whether it would work. Could I add the 6 base barcode at the 5' of my forward primers? In that case, I would be able to know which sample the sequence is from just by looking at the reading from the first 6 bases? Does anyone know why illumina design the third run of sequencing just to get the sequence of the barcodes? Thanks.
Jim
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