Has anyone tried running beyond the 2x250bp "official" capacity of the MiSeq? We worked with a sequencing collaborator who hacked together a 2x250 run before the reagents were available by unofficially (although aided by Illumina tech support) pooling a 2x150 and a 1x100 cartridge. Any idea if pooling of a 2x250 and a 2x150 is possible, allowing early access to the supposedly upcoming 2x400 run?
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
2 x 250 runs are not stably working (in our hands) as yet. Perhaps others have had better luck. No problems getting sequence, it is the decline in quality over the later cycles.
Hopefully this is just teething troubles that will get sorted out over time.
If you have the time (and money) to try the 2 x 400, go right ahead. Let us know what happens.
Comment
-
Originally posted by GenoMax View Post2 x 250 runs are not stably working (in our hands) as yet. Perhaps others have had better luck. No problems getting sequence, it is the decline in quality over the later cycles.
--
Phillip
Comment
-
Originally posted by pmiguel View PostIs this with a hardware-upgraded instrument?
Does going to lower loading densities appear to help?
--
Phillip
We are working with illumina at the moment. This may be a specific issue with our instrument. We shall see.
Comment
Latest Articles
Collapse
-
by seqadmin
While isolating and preparing single cells for sequencing was historically the bottleneck, recent technological advancements have shifted the challenge to data analysis. This highlights the rapidly evolving nature of single-cell sequencing. The inherent complexity of single-cell analysis has intensified with the surge in data volume and the incorporation of diverse and more complex datasets. This article explores the challenges in analysis, examines common pitfalls, offers...-
Channel: Articles
06-06-2024, 07:15 AM -
-
by seqadmin
Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.
Somatic Genomics
“We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...-
Channel: Articles
05-24-2024, 01:16 PM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, Yesterday, 06:58 AM
|
0 responses
13 views
0 likes
|
Last Post
by seqadmin
Yesterday, 06:58 AM
|
||
Started by seqadmin, 06-06-2024, 08:18 AM
|
0 responses
20 views
0 likes
|
Last Post
by seqadmin
06-06-2024, 08:18 AM
|
||
Started by seqadmin, 06-06-2024, 08:04 AM
|
0 responses
18 views
0 likes
|
Last Post
by seqadmin
06-06-2024, 08:04 AM
|
||
Started by seqadmin, 06-03-2024, 06:55 AM
|
0 responses
13 views
0 likes
|
Last Post
by seqadmin
06-03-2024, 06:55 AM
|
Comment