- Add 576 μl of HT1 to the DAL tube.
- Transfer 24 μl of PAL to the DAL tube containing HT1. Using the same tip, pipette up and down 3–5 times to rinse the tip and ensure complete transfer.
The recommended volumes for diluting PAL with HT1 represents a 25-fold
dilution. This dilution ratio was established by using the recommended
equipment (e.g. plate shaker calibrated for shaking speed) and following the
normalization procedure strictly under typical laboratory conditions (e.g. 20°–
25°C). If cluster density is found to be too high or too low, you may change
this dilution ratio to better suit the equipment, temperature, and user handling
in your laboratory after validation.
- Transfer 24 μl of PAL to the DAL tube containing HT1. Using the same tip, pipette up and down 3–5 times to rinse the tip and ensure complete transfer.
The recommended volumes for diluting PAL with HT1 represents a 25-fold
dilution. This dilution ratio was established by using the recommended
equipment (e.g. plate shaker calibrated for shaking speed) and following the
normalization procedure strictly under typical laboratory conditions (e.g. 20°–
25°C). If cluster density is found to be too high or too low, you may change
this dilution ratio to better suit the equipment, temperature, and user handling
in your laboratory after validation.
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