pmiguel, do you think spike in with 5% PhiX library works for Ktu application because he is using a custom sequencing primer which will not sequence from the PhiX library. The effect of PhiX spike-in in this case is just lower the cluster density his transposon library.
Besides, if you still have to spike-in >5% of high complexity library or run a control lane then "the issue" is not completely solved, is it?
Cheers,
Besides, if you still have to spike-in >5% of high complexity library or run a control lane then "the issue" is not completely solved, is it?
Cheers,
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