Hi There,
We're trying to recreate the following non-sequencing approach to evaluating GC bias with a few different polymerases. Does anyone have any experience doing so?
Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries
Aird, MG et al. 2011. Genome Biology, 12:R18 doi:10.1186/gb-2011-12-2-r18
*Corresponding author: Andreas Gnirke [email protected]
http://genomebiology.com/2011/12/2/R18/abstract
We're trying to recreate the following non-sequencing approach to evaluating GC bias with a few different polymerases. Does anyone have any experience doing so?
Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries
Aird, MG et al. 2011. Genome Biology, 12:R18 doi:10.1186/gb-2011-12-2-r18
*Corresponding author: Andreas Gnirke [email protected]
http://genomebiology.com/2011/12/2/R18/abstract