We have a few RNA samples (15) that I'm getting ready to send out to a sequencing core for some HiSeq. My question is this- are there any methods of removal of DNA that are preferred by sequencing centers? Right now my options are an on-column Qiagen prep or Promega RQ1 DNase.
Paranoia at its finest right now I think. At the stage of wanting all of these samples to be perfect prior to submission.
EDIT- I should add that the RNA was extracted using an acid-phenol methodology.
Paranoia at its finest right now I think. At the stage of wanting all of these samples to be perfect prior to submission.
EDIT- I should add that the RNA was extracted using an acid-phenol methodology.