Hi,
Recently, I have been using Miseq. I have noticed that fastq header is missing index read that Hiseq/GAII had it. For example:
@SIM:1:FCX:1:15:6329:1045 1:N:0:2
TCGCACTCAACGCCCTGCATATGACAAGACAGAATC
+
<>;##=><9=AAAAAAAAAA9#:<#<;<<<????#=
It seems that index sequence is replaced by sample number. When I ran 4 barcoded samples (indexes 4,6,8, and 12), I get something like followings:
1:N:0:1
1:N:0:2
1:N:0:3
1:N:0:4
Is there a way to make software print index sequences instead of sample number. What is the reason for this change. To make files smaller? Thank you.
Recently, I have been using Miseq. I have noticed that fastq header is missing index read that Hiseq/GAII had it. For example:
@SIM:1:FCX:1:15:6329:1045 1:N:0:2
TCGCACTCAACGCCCTGCATATGACAAGACAGAATC
+
<>;##=><9=AAAAAAAAAA9#:<#<;<<<????#=
It seems that index sequence is replaced by sample number. When I ran 4 barcoded samples (indexes 4,6,8, and 12), I get something like followings:
1:N:0:1
1:N:0:2
1:N:0:3
1:N:0:4
Is there a way to make software print index sequences instead of sample number. What is the reason for this change. To make files smaller? Thank you.
Comment