Illumina fluorophore excitation/emission spectra

[Igor]

Hi all,

I cannot seem to find what fluorophores exactly Illumina uses to label dNTPs and/or what their excitation/emission spectra are. I'd appreciate any help.

Thanks,
Igor.

[ScottC]

Have you asked their technical support?

[scifi_usa]

Excitation and Emission spectra

Were you able to find out Illumina fluorophore's excitation and emission wavelengths?

[dakota]

why are you looking for this? i'm sure their tech support can help. keep in mind there's not a lot of options given the limited visible spectral space available.

[ECO]

The odds that they tell you anything other than Em/Ex maxima are slim to none. They are likely proprietary dyes...

[GW_OK]

Here's a good starting patent for Illumina, which points tooooo

This one which talks about the dyes themselves, I think.

The description of that patent says, among other things:

Particular excitation wavelengths are 532 nm, 630 nm to 700 run, particularly 660 nm.

Lots more info as well. Good sleepy time reading.