we used exactly same PAL (stopped running) for next new running and it works well. This indicates the PAL is not a problem. it is fluidics.. illumina techsupport said "Miseq should have alert before start running, if there is flow cell problem."
But I don't think, the fluid is delivered from sample load tube by sipper 17 (miseq cartridge in position 17) for flow rate testing. if the fluid tube or sipper has air bubble or dust, the PAL sample won't be able to deliver to the flow cell chamber. It will lead to stop the premature running.
But I don't think, the fluid is delivered from sample load tube by sipper 17 (miseq cartridge in position 17) for flow rate testing. if the fluid tube or sipper has air bubble or dust, the PAL sample won't be able to deliver to the flow cell chamber. It will lead to stop the premature running.
Comment