Hi,
I experienced first problems with our MiSeq. This morning with new run loaded there was a flow rate error - first thought, valve.....
Did re-test at 250ul (failed) and 500ul (machine freezed) so had to restart. While restart I re-seated the flow cell and I managed to pass the flow rate check (no other issues). After 1h run stopped - error msg that was thrown is related to "no clusters detected". We managed to do couple runs on it with no problems (300 cycle / 500 cycle / nano and regulars). This is updated v2 version (with recently updated software).
Is this a prelude to valve problems (already reported in our collaborators lab) ?
Thanks!
I experienced first problems with our MiSeq. This morning with new run loaded there was a flow rate error - first thought, valve.....
Did re-test at 250ul (failed) and 500ul (machine freezed) so had to restart. While restart I re-seated the flow cell and I managed to pass the flow rate check (no other issues). After 1h run stopped - error msg that was thrown is related to "no clusters detected". We managed to do couple runs on it with no problems (300 cycle / 500 cycle / nano and regulars). This is updated v2 version (with recently updated software).
Is this a prelude to valve problems (already reported in our collaborators lab) ?
Thanks!
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