[pmiguel]

Do you see this on every flowcell? All the way down to the images like Phillip posted above?



The bubbles were not there in the images from the two flowcells I looked at. I will check with the lab folks here to see if they have anything to add.

Could you see what was causing the high error rates in the tiles you looked at?

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Phillip

[GW_OK]

Do you see this on every flowcell? All the way down to the images like Phillip posted above?

The bubbles were not there in the images from the two flowcells I looked at. I will check with the lab folks here to see if they have anything to add.

Not always. Very intermittent, but it does exist. And I can find those tiles and see the dark bubbles circles as Phillip showed. I'd go find some right now but we dump thumbnails after the run and I don't have anything running right now. I will say I haven't seen it in any of the rapid runs we've done so far.

[pmiguel]

My instrument is a HiSeq2500 (upgraded from a HiSeq2000). What instruments do you guys have?

[GW_OK]

Same here.

[GenoMax]

Both HiSeq 2000 and 2500.

[pmiguel]

Illumina tech support just mentioned that there is some bubble-related issue with "swath and tile drop out" at some HiSeq 2500 sites. They have launched an internal investigation.

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Phillip