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Thanks! It's super expired (back in December), but I don't have anything to lose but time and if this method works, it should be a much cheaper way to sequence amplicons than the Caporaso, multiple-primer approach. -
I've tried using expired kits before and the instrument does let you run them, but the read quality does seem to be a bit worse than a new kit. If you have nothing to lose other than a few hours of your time, I would just try it and see how things go.Leave a comment:
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Expired MiSeq reagent kit
Has anyone tried to run an expired MiSeq reagent kit? I've been working on developing an amplicon sequencing protocol, and the libraries look good through the KAPA quantification. Now I want to test them on the MiSeq without spending $1000 on a kit. We have an expired kit that we can't use for an actual, paying investigator so I wanted to at least try it out on my amplicons. Does anyone know if there's an expiration date built into the RFID tag? Our FAS didn't know.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Yesterday, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
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