Anyone else noticing a recent increase in failed Illumina Read 2 Resynthesis?

[sawilli2]

Hi All,
My company has been noticing an increase in failures of our Illumina HiSeq sequencing runs specifically in the 2nd read coming out of read 2 resynthesis. It seems that the intensities and the Q30 scores are plummeting right out of the gate after adding the read 2 reagents. We thought it was because the HiSeq uses RMX enzyme which is less stable than the MiSeq RMR enzyme but we have since replaced the RMX with RMR and are still encountering this issue. Has anyone else been experiencing this Illumina run failure type recently or is it just us?
Thanks!

[tab]

Has anyone else been seeing this issue recently?

Sawilli2, did you resolve this issue?

Thanks for any input,

[AgNewBie]

Failing read2

Hi Sawili2, Just wondering if and how you resolved the issue. I'm having a same problem recently. HiSeq 2000, single indexed, paired-end sequencing with read2 intensity and Q30 plummeting while read1 seem to be normal.