Hi Guys!
I am new to Illumina sequencing and I have a sample pretty much ready to go containing around 1ug of genomic DNA. I checked the quantity using agarose gel comparing the genomic band to a quantitative ladder and another quantitative marker. The 260/280 ratio is 1.8 but the 260/230 ratio is only 1.2
Will this be detrimental to a run with a MiSeq?
Thanks in advance!
I am new to Illumina sequencing and I have a sample pretty much ready to go containing around 1ug of genomic DNA. I checked the quantity using agarose gel comparing the genomic band to a quantitative ladder and another quantitative marker. The 260/280 ratio is 1.8 but the 260/230 ratio is only 1.2
Will this be detrimental to a run with a MiSeq?
Thanks in advance!
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