Hello,
The newest version of the XT kit does not include the suggestions to do QC along the way anywhere - which makes me very nervous.
The older protocol had you check via a BioAnalyzer chip for the size distribution, and quantify with Qubit.
Now they just have the library normalization step with the beads, does anyone have experience with this step - is it consistently successful? I have a hard time blindly trusting the protocol!
Any information or experiences you've had would be very helpful
The newest version of the XT kit does not include the suggestions to do QC along the way anywhere - which makes me very nervous.
The older protocol had you check via a BioAnalyzer chip for the size distribution, and quantify with Qubit.
Now they just have the library normalization step with the beads, does anyone have experience with this step - is it consistently successful? I have a hard time blindly trusting the protocol!
Any information or experiences you've had would be very helpful
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